Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth . In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site . In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 . Its activity is dependent on its phosphorylation state and binding to 14-3-3 . May also play a role in nerve growth factor-mediated neuroprotection (By similarity). .

Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate),AKT1S1 PRAS40

Q96B36,Q9D1F4

84335/67605

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,ELISA

WB 1:1000-5000
IHC 1:100-400
IF 1:100-1:400
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):LNtSD

40kDa

Rabbit

Monoclonal

F302

IgG,Kappa

Protein A

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Store at -20℃. Stable for 12 months from date of receipt.