Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor . Recognizes the substrate consensus sequence [ST]-Q . Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis . Phosphorylates ''Ser-139'' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism . Required for FANCD2 ubiquitination . Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication . Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 . Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress . Acts as a mechanical stress sensor at the nuclear envelope: relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 . Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at ''Ser-282'', leading to lamin disassembly . Involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at ''Ser-395'', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture . The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity . Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity). .

Serine/threonine-protein kinase ATR (EC 2.7.11.1) (Ataxia telangiectasia and Rad3-related protein) (FRAP-related protein 1),ATR FRP1

Q13535,Q9JKK8

545

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,ELISA

WB 1:1000-1:5000
IHC 1:200-1:1000
IF 1:100-1:400
ICC 1:100-1:400
IP 1:50-1:200

Endogenous

301kDa

Rabbit

Monoclonal

H942

IgG,Kappa

Protein A

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Store at -20℃. Stable for 12 months from date of receipt.