Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor . Recognizes the substrate consensus sequence [ST]-Q . Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis . Phosphorylates ''Ser-139'' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism . Required for FANCD2 ubiquitination . Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication . Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 . Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress . Acts as a mechanical stress sensor at the nuclear envelope: relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 . Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at ''Ser-282'', leading to lamin disassembly . Involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at ''Ser-395'', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture . The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity . Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity). .

Serine/threonine-protein kinase ATR (EC 2.7.11.1) (Ataxia telangiectasia and Rad3-related protein) (FRAP-related protein 1),ATR FRP1

Q13535,Q9JKK8

545

Human,Mouse,Rat

WB,IHC,IF,ICC,ELISA

WB 1:500-1:2000
IHC 1:100-400
IF 1:100-1:400
ICC 1:100-1:400
ELISA 1:5000-1:20000

ATR (Phospho Ser428) Antibody detects endogenous levels of ATR protein only when phosphorylated at S428.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GIsPK

290kDa

Rabbit

Monoclonal

L265

IgG,Kappa

Protein A

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Store at -20℃. Stable for 12 months from date of receipt.