Functions both as NADH oxidoreductase and as regulator of apoptosis . In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway . Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces ''parthanatos'' i.e. caspase-independent fragmentation of chromosomal DNA . Binds to DNA in a sequence-independent manner . Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis . Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells . In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import . .; [Isoform 4]: Has NADH oxidoreductase activity. Does not induce nuclear apoptosis. .; [Isoform 5]: Pro-apoptotic isoform. .

o95831

Apoptosis-inducing factor 1, mitochondrial (EC 1.6.99.-) (Programmed cell death protein 8),AIFM1 AIF PDCD8,AIFM1,AIF,Programmed cell death protein 8,PDCD8,Apoptosis-inducing factor 1,mitochondrial

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

67kD

Rabbit

Recombinant Human AIFM1/AIF (Leu102-Asp613).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.