Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins . Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome . By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication . May be implicated in the shutoff of protein synthesis through its interactions with components of the host translation machinery . Blocks host RELA nuclear translocation upon cytokine stimulation, thereby impairing NF-kappa-B signaling . Inhibits type I IFN production and ''Lys-63''-linked polyubiquitination of host TBK1 through binding to host RNF138. This binding enhances the interaction between RNF138 and RNF128 and promotes RNF138-mediated degradation of RNF128 . Also inhibits the activation of AP-1 transcription factor . Monoubiquitinates the viral protein P15/PIG1 in vitro . .

p27949

Ubiquitin-conjugating enzyme E2 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme) (UBCv1) (Ubiquitin carrier protein) (Ubiquitin-protein ligase) (pI215L),UBC BA71V-144 I215L,Ubiquitin-conjugating enzyme E2, 2.3.2.23, E2 ubiquitin-conjugating enzyme, UBCv1, Ubiquitin carrier protein, Ubiquitin-protein ligase, pI215L, UBC, BA71V-144, I215L

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

25kD

Rabbit

Recombinant ASFV UBC/pI215L (Met1-Thr200).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.