Lethal factor (LF), which constitutes one of the three proteins composing the anthrax toxin, is able to trigger rapid cell death in macrophages . Acts as a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5): cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes . Also cleaves mouse Nlrp1b: host Nlrp1b cleavage promotes ubiquitination and degradation of the N-terminal part of Nlrp1b by the proteasome, thereby releasing the cleaved C-terminal part of Nlrp1b, which polymerizes and forms the Nlrp1b inflammasome followed by host cell pyroptosis . Able to cleave mouse Nlrp1b alleles 1 and 5, while it is not able to cleave Nlrp1b alleles 2, 3 and 4 . In contrast, does not cleave NLRP1 human ortholog . LF is not toxic by itself and only acts as a lethal factor when associated with protective antigen (PA) to form the lethal toxin (LeTx): PA is required for LF translocation into the host cytosol . .

p15917

Lethal factor (LF) (EC 3.4.24.83) (Anthrax lethal toxin endopeptidase component),lef pXO1-107 BXA0172 GBAA_pXO1_0172,lef, LF, Lethal factor, Anthrax lethal toxin endopeptidase component, Bacillus anthracis

Bacillus anthracis

IP,ELISA,SPR

IP 1:50-1:200
ELISA 1:5000-1:20000
SPR,The optimal dilutions should be determined by the end user.

94kD

Mouse

Monoclonal

F297

IgG2a

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.