Enzyme that can both act as a NAD(P)H-dependent reductase and a S-nitroso-CoA-dependent nitrosyltransferase . Promotes fetal heme degradation during development . Also expressed in adult tissues, where it acts as a regulator of hematopoiesis, intermediary metabolism (glutaminolysis, glycolysis, TCA cycle and pentose phosphate pathway) and insulin signaling . Has a broad specificity oxidoreductase activity by catalyzing the NAD(P)H-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone) . Contributes to fetal heme catabolism by catalyzing reduction of biliverdin IXbeta into bilirubin IXbeta in the liver . Biliverdin IXbeta, which constitutes the major heme catabolite in the fetus is not present in adult . Does not reduce bilirubin IXalpha . Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH . Acts as a protein nitrosyltransferase by catalyzing nitrosylation of cysteine residues of target proteins, such as HMOX2, INSR and IRS1 . S-nitroso-CoA-dependent nitrosyltransferase activity is mediated via a ''ping-pong'' mechanism: BLVRB first associates with both S-nitroso-CoA and protein substrate, nitric oxide group is then transferred from S-nitroso-CoA to Cys-109 and Cys-188 residues of BLVRB and from S-nitroso-BLVRB to the protein substrate . Inhibits insulin signaling by mediating nitrosylation of INSR and IRS1, leading to their inhibition . .

p30043

Flavin reductase (NADPH) (FR) (EC 1.5.1.30) (Biliverdin reductase B) (BVR-B) (EC 1.3.1.-) (Biliverdin-IX beta-reductase) (Green heme-binding protein) (GHBP) (NADPH-dependent diaphorase) (NADPH-flavin reductase) (FLR) (S-nitroso-CoA-assisted nitrosyltransferase) (SNO-CoA-assisted nitrosyltransferase) (EC 2.6.99.-),BLVRB FLR SCAN,FR,Green heme-binding protein,BLVRB,Flavin reductase (NADPH),FLR,NADPH-flavin reductase,GHBP,Biliverdin-IX beta-reductase,NADPH-dependent diaphorase,BVR-B,Biliverdin reductase B

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

23kD

Rabbit

Recombinant Human BLVRB (Ala2-Gln206).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.