[N-terminal protease]: Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation . .; [Capsid protein C]: Packages viral RNA to form a viral nucleocapsid and thereby protects viral RNA. Also plays a role in transcription regulation. Protects the incoming virus against IFN-induced effectors. .; [E(rns) glycoprotein]: Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans . Also possesses intrinsic ribonuclease (RNase) activity that can inhibit the production of type I interferon and assist in the development of persistent infections (By similarity). .; [Envelope glycoprotein E1]: E1 and/or E2 are probably responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis. .; [Envelope glycoprotein E2]: E1 and/or E2 are probably responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (Probable). Probably functions as a coeffector of fusion providing structural integrity to the fusion complex and possibly controlling exposure of the fusion motif in E1 . .; [Viroporin p7]: Plays an essential role in the virus replication cycle by acting as a viroporin (By similarity). Forms ion conductive pores, which alters the cell permeability allowing the transport of ions and other small molecules (By similarity). Forms a leader sequence to properly orient NS2 in the membrane. .; [Non-structural protein 2-3]: Uncleaved NS2-3 is required for production of infectious virus. .; [Cysteine protease NS2]: Plays a role in the regulation of viral RNA replication. .; [Serine protease NS3]: Multifunctional protein that contains an N-terminal protease and a C-terminal helicase, playing essential roles in viral polyprotein processing and viral genome replication. The chymotrypsin-like serine protease activity utilizes NS4A as an essential cofactor and catalyzes the cleavage of the polyprotein leading to the release of NS4A, NS4B, NS5A, and NS5B. Interacts with NS5B to enhance RNA-dependent RNA polymerase activity. .; [Non-structural protein 4A]: Acts as a cofactor for the NS3 protease activity. .; [Non-structural protein 4B]: Induces a specific membrane alteration that serves as a scaffold for the virus replication complex (By similarity). Plays a role in the inhibition of host innate immune response by inhibiting RIGI/IFIH1-mediated IFN-beta production . .; [RNA-directed RNA polymerase]: Replicates the viral (+) and (-) genome. Initiates the primer-independent RNA replication via a de novo mechanism requiring GTP . .

p19711

Genome polyprotein [Cleaved into: N-terminal protease (N-pro) (EC 3.4.22.-) (Autoprotease p20); Capsid protein C (p14); E(rns) glycoprotein (gp44/48) (EC 4.6.1.19); Envelope glycoprotein E1 (gp33); Envelope glycoprotein E2 (gp55); Viroporin p7; Non-structural protein 2-3; Cysteine protease NS2 (EC 3.4.22.-) (Non-structural protein 2) (p54); Serine protease NS3 (EC 3.4.21.113) (EC 3.6.1.15) (EC 3.6.4.13) (Non-structural protein 3) (p80); Non-structural protein 4A (NS4A) (p10); Non-structural protein 4B (NS4B) (p30); Non-structural protein 5A (NS5A) (p58); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5B) (p75)],Genome polyprotein, E(rns) glycoprotein, gp44/48

Bovine viral diarrhea virus (isolate NADL) (BVDV) (Mucosal disease virus)

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

450kD

Rabbit

Recombinant BVDV E(rns)/gp44/48 (Glu271-Ala497).

Polyclonal

IgG

Antigen affinity purification.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.