[N-terminal protease]: Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation. .; [Capsid protein C]: Packages viral RNA to form a viral nucleocapsid and thereby protects viral RNA. Also plays a role in transcription regulation. Protects the incoming virus against IFN-induced effectors. .; [E(rns) glycoprotein]: Plays a role in viral entry. Interacts with host RPSA that acts as a cellular attachment receptor for the virus. Also possesses intrinsic ribonuclease (RNase) activity that can inhibit the production of type I interferon and assist in the development of persistent infections. Cleaves preferentially NpU bonds. Binds to heparan sulfate on the host cells for entry . .; [Envelope glycoprotein E1]: Plays a role in cell attachment and subsequent fusion of viral and cellular membranes. Therefore, mediates together with envelope glycoprotein E2 the viral entry. .; [Envelope glycoprotein E2]: Plays a role in cell attachment and subsequent fusion of viral and cellular membranes (By similarity). Therefore, mediates together with envelope glycoprotein E1 the viral entry (By similarity). Binds to host ADAM17 receptor for entry (By similarity). Binds to host ANXA2 for entry . Binds to host MERTK for entry . .; [Viroporin p7]: Plays an essential role in the virus replication cycle by acting as a viroporin. Forms ion conductive pores, which alters the cell permeability allowing the transport of ions and other small molecules. .; [Non-structural protein 2-3]: Autoprotease that associates with the host chaperone JIV and cleaves the NS2-3 protein between NS2 and NS3. Also plays a role in the formation of infectious particles. .; [Cysteine protease NS2]: Plays a role in the regulation of viral RNA replication. .; [Serine protease NS3]: Multifunctional protein that contains an N-terminal protease and a C-terminal helicase, playing essential roles in viral polyprotein processing and viral genome replication. The chymotrypsin-like serine protease activity utilizes NS4A as an essential cofactor and catalyzes the cleavage of the polyprotein leading to the release of NS4A, NS4B, NS5A, and NS5B. Plays a role in the inhibition of host NF-kappa-B activation by interacting with and inhibiting host TRAF6. Interacts with NS5B to enhance RNA-dependent RNA polymerase activity. .; [Non-structural protein 4A]: Acts as a cofactor for the NS3 protease activity. .; [Non-structural protein 4B]: Induces a specific membrane alteration that serves as a scaffold for the virus replication complex (By similarity). Antagonizes host cell apoptosis by interacting with host ferritin heavy chain. The ORF4 protein physically binds host FTH1/FHC, resulting in the reduction of FTH1 protein levels in host cells. Reduction of FTH1 concentration further inhibits the accumulation of reactive oxygen in host cells, leading to reduced apoptosis. .; [Non-structural protein 5A]: Regulates viral RNA replication by interacting with the 3''-untranslated region of viral RNA in a dose-dependent manner. At small concentrations promotes viral synthesis by interacting with the polymerase NS5B while at large concentrations, inhibits replication. .; [RNA-directed RNA polymerase]: Replicates the viral (+) and (-) genome. .

p21530

Genome polyprotein [Cleaved into: N-terminal protease (N-pro) (EC 3.4.22.-) (Autoprotease p20); Capsid protein C; E(rns) glycoprotein (gp44/48); Envelope glycoprotein E1 (gp33); Envelope glycoprotein E2 (gp55); Viroporin p7; Non-structural protein 2-3 (NS2-3); Cysteine protease NS2 (EC 3.4.22.-) (Non-structural protein 2); Serine protease NS3 (EC 3.4.21.113) (EC 3.6.1.15) (EC 3.6.4.13) (Non-structural protein 3); Non-structural protein 4A (NS4A); Non-structural protein 4B (NS4B); Non-structural protein 5A (NS5A); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5B)],Genome polyprotein, Envelope glycoprotein E2, gp55

Classical swine fever virus (CSFV)

ELISA

ELISA 1:5000-1:20000

439kD

Mouse

Monoclonal

SA467

VHH-mFc

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.