Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination . Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3''-triiodothyronine (T3), 3,3'',5''-triiodothyronine (rT3) to 3,3''-diiodothyronine (3,3''-T2) and 3'',5''-diiodothyronine (3'',5''-T2) to 3''-monoiodothyronine (3''-T1) via outer-ring deiodination (ORD) . Catalyzes the deiodination of T4 to 3,3'',5''-triiodothyronine (rT3) via inner-ring deiodination (IRD) . Catalyzes the deiodination of T3 to 3,3''-T2, 3,5-diiodothyronine (3,5-T2) to 3- monoiodothyronine (3-T1) and 3,3''-T2 to 3-T1 via IRD (By similarity). Catalyzes the phenolic ring deiodinations of 3,3'',5''-triiodothyronamine and 3'',5''-diiodothyronamine . Catalyzes the phenolic ring deiodination of 3,3''-diiodothyronamine and tyrosyl ring deiodinations of 3,5,3''-triiodothyronamine and 3,5-diiodothyronamine (By similarity). Catalyzes the deiodination of L-thyroxine sulfate and 3,3'',5-triiodo-L-thyronine sulfate via IRD and of 3,3'',5''-triiodo-L-thyronine sulfate via ORD (By similarity). .

p49895

Type I iodothyronine deiodinase (EC 1.21.99.3) (EC 1.21.99.4) (5DI) (DIOI) (Type 1 DI) (Type-I 5''-deiodinase),DIO1 ITDI1 TXDI1,Type I iodothyronine deiodinase, 1.21.99.4, 5DI, DIOI, Type 1 DI, Type-I 5''-deiodinase, DIO1, ITDI1, TXDI1

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

29kD

Rabbit

Recombinant Human DIO1 (Gly89-Ser254).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.