Transcription factor that acts as a molecular switch to induce the transition from the latent to the lytic or productive phase of the virus cycle (Probable) . Mediates the switch from the latent to the lytic cycle of infection in cells containing a highly methylated viral genome . Probably binds to silenced chromatin and recruits host chromatin-remodeling enzymes . Regulates this switch by binding to 2 types of ZEBRA response elements (ZREs): the CpG-free AP-1 like elements (latency) and the methylated CpG-containing elements (lytic replication) (Probable) . Activates preferentially the methylated forms of the viral lytic R (BRLF1) and Na (BRRF1) gene promoters, the latters being the first genes activated during Z-mediated reactivation in latently infected cells . BZLF1 and BRLF1 act together to trigger lytic replication . Also binds the lytic origin of replication, oriLyt . Induces G1 cell cycle arrest by stabilizing the host CCAAT/enhancer binding protein CEBPA . This function is important because the lytic cycle preferentially takes place in host cells arrested in G1 . .

p03206

Lytic switch protein BZLF1 (EB1) (Protein Z) (Trans-activator protein BZLF1) (Zta) (bZIP transcription factor ZEBRA),BZLF1,Lytic switch protein BZLF1, EB1, Protein Z, Trans-activator protein BZLF1, Zta, bZIP transcription factor ZEBRA, BZLF1

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

27kD

Rabbit

Recombinant EBV/HHV-4 BZLF1 (Leu175-Phe245).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.