Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection . EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency . EBNA1 binding to DS recruits the host origin recognition complex (ORC) . Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes . Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes . Counteracts the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival . Induces degradation of host PML through the ubiquitin-proteasome system, which promotes lytic reactivation and may impair the host cell DNA repair . Increases the association of CK2 with PML proteins which increases the phosphorylation of PML proteins by CK2, triggering the polyubiquitylation and degradation of PML . Displays inhibitory effects on a SUMO2-modified complex that includes STUB1, KAP1 and USP7 . This inhibitory effect possibly participates to the maintenance of latency linked to PML silencing . .

p03211

Epstein-Barr nuclear antigen 1 (EBNA-1) (EBV nuclear antigen 1) (EC 3.1.21.-),EBNA1 BKRF1,Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

WB,IF,ICC,ELISA

WB 1:500-1:2000
IF 1:100-1:500
ICC 1:100-1:400
ELISA 1:5000-1:20000

57kD

Mouse

Monoclonal

D280

IgG2a, kappa

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.