Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
An inverse autotransporter . Adhesin, which mediates attachment to the human intestine epithelial cells . Necessary for the production of attaching and effacing lesions on infected human tissue culture cells . Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable). Periplasmic domain binds purified E.coli PGN sacculi under acidic conditions in vitro and in vivo, but does not bind to chitin . Periplasmic domain binds PGN sacculi with an apparent dissociation constant (Kd) of 0.8 uM . No binding to PGN in vitro at normal physiological pH 7.4 . .
p19809
Intimin (Attaching and effacing protein) (Eae protein),eae eaeA E2348C_3939,Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
WB,IHC,ELISA
WB 1:500-1:2000IHC 1:50-1:300ELISA 1:5000-1:20000
103kD
Rabbit
Recombinant Escherichia coli eae/Intimin (Lys450-Gln655).
Polyclonal
IgG
Purified by antigen affinity column.
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.
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