The large envelope protein exists in two topological conformations, one which is termed ''external'' or Le-HBsAg and the other ''internal'' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. .; The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. .

p03141

Large envelope protein (L glycoprotein) (L-HBsAg) (LHB) (Large S protein) (Large surface protein) (Major surface antigen),S,Large envelope protein S, L glycoprotein, L-HBsAg, LHB, Large surface protein, Major surface protein, preS1 domain, Pre-S1

Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A)

FC,SPR

FC 1:50-1:200
SPR,The optimal dilutions should be determined by the end user.

44kD

Human

Monoclonal

G256

IgG1

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1.95 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.