Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) . Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring . May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression . Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at ''Ser-10'' of histone H3; in this function may act redundantly with AKAP8L . Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation . May be involved in regulation of DNA replication by acting as scaffold for MCM2 . Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 ''Lys-4'' methylation . May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells . May act as a carrier protein of GJA1 for its transport to the nucleus . May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions . Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity). .

o43823

A-kinase anchor protein 8 (AKAP-8) (A-kinase anchor protein 95 kDa) (AKAP 95),AKAP8 AKAP95,AKAP8, A-kinase anchor protein 8, AKAP95, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

77kD

Rabbit

Recombinant Human AKAP8 (Met1-Glu572).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.