E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation . Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) . The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane . In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination . The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction . In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at ''Cys-277'', leading to its degradation when the lipid levels are low . Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols . Mediates polyubiquitination of INSIG2 at ''Cys-215'' in some tissues, leading to its degradation . Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex . Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation . Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor . In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 . Regulates NF-kappa-B and MAPK signaling pathways by mediating ''Lys-27''-linked polyubiquitination of TAB3 and promoting subsequent TAK1/MAP3K7 activation . Required for proper lipid homeostasis . .

q9ukv5

E3 ubiquitin-protein ligase AMFR, 2.3.2.36, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45,AMFR

Mus musculus, Homo sapiens

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

73kD

Rabbit

Recombinant Human AMFR (Gln456-Ser643).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.