May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics . May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation . Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 . Required for the colocalization of VAMP7 and Rab21, probably on TGN sites . Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 . Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). .

q96nw4

Ankyrin repeat domain-containing protein 27 (VPS9 domain-containing protein),ANKRD27 PP12899,ANKRD27, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

117kD

Rabbit

Recombinant Human ANKRD27 (Met654-Ser902).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.