Functions as a key mediator in apoptosis and inflammation . Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner . Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3 . Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines . Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death . The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions . Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization . In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1 . In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation . May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation . In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8 . In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form . Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways . For regulation of NF-kappa-B activating and inhibiting functions have been reported . Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK . Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing . Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA . .; [Isoform 2]: May have a regulating effect on the function as inflammasome adapter. .; [Isoform 3]: Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. .

q9ulz3

Apoptosis-associated speck-like protein containing a CARD (hASC) (Caspase recruitment domain-containing protein 5) (PYD and CARD domain-containing protein) (Target of methylation-induced silencing 1),PYCARD ASC CARD5 TMS1,TMS1, CARD5, Caspase recruitment domain-containing protein 5, Apoptosis-associated speck-like protein containing a CARD, PYD and CARD domain-containing protein, ASC, PYCARD, hASC, Target of methylation-induced silencing 1

Human

IF,ICC,IP,ELISA

IF 1:100-1:500
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

22kD

Alpaca

Monoclonal

SA433

VHH-8His-Cys-tag

Purified by Nickel column.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.