Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades . The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state . Signaling by an activated GPCR promotes GDP release and GTP binding . The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal . Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins . Signaling is mediated via effector proteins, such as adenylate cyclase: inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels . The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis . Required for cortical dynein-dynactin complex recruitment during metaphase . .

p63096 & p62873

Guanine nucleotide-binding protein G(i) subunit alpha-1 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein),GNAI1,GNAI1, Guanine nucleotide-binding protein G(i) subunit alpha-1, Adenylate cyclase-inhibiting G alpha protein, Gi1 protein alpha subunit, G protein subunit alpha i1, Gαi1GNB1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1, G protein subunit beta 1, Gβ1

Human

IP,ELISA

IP 1:50-1:200
ELISA 1:5000-1:20000

41kD

Mouse

Monoclonal

mA262

IgG2a, kappa

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.