Exhibits adenosine 5''-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5''monophosphoramidate (AMP-NH2) to yield AMP and NH2 . Hydrolyzes adenosine 5''monophosphomorpholidate (AMP-morpholidate) and guanosine 5''monophosphomorpholidate (GMP-morpholidate) . Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester . Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro . Can also convert adenosine 5''-O-phosphorothioate and guanosine 5''-O-phosphorothioate to the corresponding nucleoside 5''-O-phosphates with concomitant release of hydrogen sulfide . In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1 . Modulates p53/TP53 levels and p53/TP53-mediated apoptosis . Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex . Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17 . Deconjugates SUMO1 from RANGAP1 (By similarity). .

p49773

Adenosine 5''-monophosphoramidase HINT1 (EC 3.9.1.-) (Desumoylating isopeptidase HINT1) (EC 3.4.22.-) (Histidine triad nucleotide-binding protein 1) (Protein kinase C inhibitor 1) (Protein kinase C-interacting protein 1) (PKCI-1),HINT1 HINT PKCI1 PRKCNH1,Histidine triad nucleotide-binding protein 1, HINT, Adenosine 5''-monophosphoramidase, HINT1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI1

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

14kD

Rabbit

Recombinant Human HINT1 (Met1-Gly126).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.