Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses . Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation . Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues . These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome . In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis . In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE . IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs . Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor . Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 . Phosphorylates RIPK1 at ''Ser-25'' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus . Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at ''Thr-749'' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses . IKBKB-mediated phosphorylation of STAT1 at ''Thr-749'' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 . It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription . .

o14920

Inhibitor of nuclear factor kappa-B kinase subunit beta (I-kappa-B-kinase beta) (IKK-B) (IKK-beta) (IkBKB) (EC 2.7.11.10) (I-kappa-B kinase 2) (IKK-2) (IKK2) (Nuclear factor NF-kappa-B inhibitor kinase beta) (NFKBIKB) (Serine/threonine protein kinase IKBKB) (EC 2.7.11.1),IKBKB IKKB,IKKB, IKK-beta, Serine/threonine protein kinase IKBKB, I-kappa-B-kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, IKBKB, IKK-B, NFKBIKB, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, IkBKB

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

87kD

Rabbit

Recombinant Human IKBKB (Glu16-Gly345).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.