Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase . Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65 . Hydroxylates its own N-terminus, which is required for homooligomerization . Plays a role in the regulation of nucleolar liquid-liquid phase separation (LLPS) by post-translationally modifying LIAT1 at its lysine-rich domain which inhibits LIAT1 nucleolar targeting (By similarity). In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA . Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation . Demethylates histone H3 at ''Arg-2'' (H3R2me) and histone H4 at ''Arg-3'' (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code . However, histone arginine demethylation may not constitute the primary activity in vivo . In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation . Demethylates other arginine methylated-proteins such as ESR1 . Has no histone lysine demethylase activity . Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65 (By similarity). Seems to be necessary for the regulation of macrophage cytokine responses . .

q6nyc1

Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (EC 1.14.11.-) (Histone arginine demethylase JMJD6) (JmjC domain-containing protein 6) (Jumonji domain-containing protein 6) (Lysyl-hydroxylase JMJD6) (Peptide-lysine 5-dioxygenase JMJD6) (Phosphatidylserine receptor) (Protein PTDSR),JMJD6 KIAA0585 PSR PTDSR,Histone arginine demethylase JMJD6, Phosphatidylserine receptor, JmjC domain-containing protein 6, JMJD6, PTDSR, PSR, Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, Jumonji domain-containing protein 6, Protein PTDSR, Peptide-lysine 5-dioxygenase JMJD6, Lysyl-hydroxylase JMJD6, KIAA0585

Human

IF,ICC,IP,ELISA

IF 1:100-1:500
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

47kD

Mouse

Monoclonal

SA449

IgG1

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.