Histone demethylase that specifically demethylates ''Lys-9'' and ''Lys-36'' residues of histone H3, thereby playing a central role in histone code . Does not demethylate histone H3 ''Lys-4'', H3 ''Lys-27'' nor H4 ''Lys-20''. Demethylates trimethylated H3 ''Lys-9'' and H3 ''Lys-36'' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. .; [Isoform 2]: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. .

o75164

Lysine-specific demethylase 4A (EC 1.14.11.66) (EC 1.14.11.69) (JmjC domain-containing histone demethylation protein 3A) (Jumonji domain-containing protein 2A) ([histone H3]-trimethyl-L-lysine(36) demethylase 4A) ([histone H3]-trimethyl-L-lysine(9) demethylase 4A),KDM4A JHDM3A JMJD2 JMJD2A KIAA0677,Lysine-specific demethylase 4A, JMJD2, Jumonji domain-containing protein 2A, KDM4A, KIAA0677, JHDM3A, [histone H3]-trimethyl-L-lysine(9) demethylase 4A, [histone H3]-trimethyl-L-lysine(36) demethylase 4A, JMJD2A, JmjC domain-containing histone demethylation protein 3A

Human

IF,ICC,IP,ELISA

IF 1:100-1:500
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

121kD

Mouse

Monoclonal

SA450

IgG2a

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.