Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) . Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5''-RAGGU-3'' motif within the variable loop . M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay . Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs . Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation . Also methylates a specific subset of miRNAs, such as let-7 . N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) . Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity). .

q9ubp6

tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33) (Methyltransferase-like protein 1) (mRNA (guanine-N(7)-)-methyltransferase) (EC 2.1.1.-) (miRNA (guanine-N(7)-)-methyltransferase) (EC 2.1.1.-) (tRNA (guanine(46)-N(7))-methyltransferase) (tRNA(m7G46)-methyltransferase),METTL1 C12orf1,miRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, tRNA (guanine-N(7)-)-methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, C12orf1, tRNA (guanine(46)-N(7))-methyltransferase, METTL1, tRNA(m7G46)-methyltransferase

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

32kD

Rabbit

Recombinant Human METTL1 (Thr34-His276).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.