Catalytic component of the GATOR1 complex, a multiprotein complex that functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway . In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling . In the presence of abundant amino acids, the GATOR1 complex is ubiquitinated and inhibited by GATOR2 . Within the GATOR1 complex, NPRL2 constitutes the catalytic subunit that mediates the GTPase activator activity and under methionine-sufficient conditions, the GTPase activator activity is inhibited by PRMT1 through methylation and consequently inducing timely mTORC1 activation . .; Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling . Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at ''Tyr-9'', ''Tyr-373'' and ''Tyr-376'' residues . May act as a tumor suppressor . Suppresses cell growth and enhances sensitivity to various anticancer drugs . .

q8wtw4

GATOR1 complex protein NPRL2 (Gene 21 protein) (G21 protein) (Nitrogen permease regulator 2-like protein) (NPR2-like protein) (Tumor suppressor candidate 4),NPRL2 TUSC4,Gene 21 protein, TUSC4, G21 protein, NPRL2, GATOR complex protein NPRL2, Tumor suppressor candidate 4, Nitrogen permease regulator 2-like protein, NPR2-like protein

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

44kD

Rabbit

Recombinant Human NPRL2 (Arg6-Glu161).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.