Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation . Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium . .; The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. .

p02818

Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein),BGLAP,Gamma-carboxyglutamic acid-containing protein, BGLAP, BGP, Bone Gla protein, Osteocalcin

Human

ELISA,Blocking

ELISA 1:5000-1:20000
Blocking,The optimal dilutions should be determined by the end user.

11kD

Mouse

Monoclonal

KT465

IgG2a, kappa

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.