Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase . Specifically demethylates ''Lys-4'' (H3K4me) and ''Lys-36'' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity). Preferentially demethylates trimethylated H3 ''Lys-4'' (H3K4me3) and monomethylated H3 ''Lys-4'' (H3K4me1) residues, while it has weaker activity for dimethylated H3 ''Lys-36'' (H3K36me2) (By similarity). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). Also catalyzes demethylation of non-histone proteins, such as CGAS: demethylation of monomethylated CGAS promotes interaction between CGAS and PARP1, followed by PARP1 inactivation (By similarity). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on ''His-216'', thereby playing a role in ribosome biogenesis . Participates in MYC-induced transcriptional activation . .

q9h6w3

Ribosomal oxygenase 1 (60S ribosomal protein L8 histidine hydroxylase) (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66) (EC 1.14.11.27, EC 1.14.11.79) (Myc-associated protein with JmjC domain) (Nucleolar protein 66) (hsNO66) (Ribosomal oxygenase NO66) (ROX),RIOX1 C14orf169 MAPJD NO66,ROX, MAPJD, C14orf169, hsNO66, Myc-associated protein with JmjC domain, Ribosomal oxygenase 1, Nucleolar protein 66, Ribosomal oxygenase NO66, Histone lysine demethylase NO66, 60S ribosomal protein L8 histidine hydroxylase, NO66, Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66, RIOX1

Human

IF,ICC,IP,ELISA

IF 1:100-1:500
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

72kD

Mouse

Monoclonal

SA440

IgG2a

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.