Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5''-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity . In parallel, has a histone methyltransferase activity and methylates ''Lys-4'' and ''Lys-36'' of histone H3. Specifically mediates dimethylation of H3 ''Lys-36'' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining . Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A . .

q53h47

Histone-lysine N-methyltransferase SETMAR (SET domain and mariner transposase fusion protein) (Metnase) [Includes: Histone-lysine N-methyltransferase (EC 2.1.1.357); Transposon Hsmar1 transposase (EC 3.1.-.-)],SETMAR,Histone-lysine N-methyltransferase SETMAR, SETMAR, SET domain and mariner transposase fusion protein, Metnase

Human

IF,ICC,IP,ELISA

IF 1:100-1:500
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

79kD

Mouse

Monoclonal

SA470

IgG2a

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.