The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription . TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) . The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 . TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly . TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2 . Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors . Phosphorylates TP53 on ''Thr-55'' which leads to MDM2-mediated degradation of TP53 . Phosphorylates GTF2A1 and GTF2F1 on Ser residues . Possesses DNA-binding activity . Essential for progression of the G1 phase of the cell cycle . Exhibits histone acetyltransferase activity towards histones H3 and H4 . .

p21675

Transcription initiation factor TFIID subunit 1 (EC 2.3.1.48) (EC 2.7.11.1) (Cell cycle gene 1 protein) (TBP-associated factor 250 kDa) (p250) (Transcription initiation factor TFIID 250 kDa subunit) (TAF(II)250) (TAFII-250) (TAFII250),TAF1 BA2R CCG1 CCGS TAF2A,p250, TAF(II)250, CCGS, BA2R, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TAFII-250, TAF2A, CCG1, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, TAFII250

Human

IF,ICC,IP,ELISA

IF 1:100-1:500
ICC 1:100-1:400
IP 1:50-1:200
ELISA 1:5000-1:20000

215kD

Mouse

Monoclonal

SA478

IgG2a

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.