RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences . Binds to U-rich sequences immediately downstream from a 5'' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing . Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5'' splice site is adjacent to IAS1 (By similarity). Activates the use of heterologous 5'' splice sites; the activation depends on the intron sequence downstream from the 5'' splice site, with a preference for a downstream U-rich sequence . By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5'' splice sites followed by U-rich sequences, thereby facilitating atypical 5'' splice site recognition by U1 snRNP . Activates splicing of alternative exons with weak 5'' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIAR mRNA (By similarity). Acts as a modulator of alternative splicing for the apoptotic FAS receptor, thereby promoting apoptosis . Binds to the 5'' splice site region of FAS intron 5 to promote accumulation of transcripts that include exon 6 at the expense of transcripts in which exon 6 is skipped, thereby leading to the transcription of a membrane-bound apoptotic FAS receptor, which promotes apoptosis . Binds to a conserved AU-rich cis element in COL2A1 intron 2 and modulates alternative splicing of COL2A1 exon 2 . Also binds to the equivalent AT-rich element in COL2A1 genomic DNA, and may thereby be involved in the regulation of transcription . Binds specifically to a polypyrimidine-rich controlling element (PCE) located between the weak 5'' splice site and the intronic splicing silencer of CFTR mRNA to promote exon 9 inclusion, thereby antagonizing PTB1 and its role in exon skipping of CFTR exon 9 . Involved in the repression of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3'' untranslated regions (3'' UTRs), including target ARE-bearing mRNAs encoding TNF and PTGS2 (By similarity). Also participates in the cellular response to environmental stress, by acting downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SGs), leading to stress-induced translational arrest . Formation and recruitment to SGs is regulated by Zn(2+) (By similarity). Possesses nucleolytic activity against cytotoxic lymphocyte target cells . .; [Isoform Short]: Displays enhanced splicing regulatory activity compared with TIA isoform Long. .

p31483

Cytotoxic granule associated RNA binding protein TIA1 (Nucleolysin TIA-1 isoform p40) (RNA-binding protein TIA-1) (T-cell-restricted intracellular antigen-1) (TIA-1) (p40-TIA-1),TIA1,TIA1, T-cell-restricted intracellular antigen-1, RNA-binding protein TIA-1, TIA-1, Nucleolysin TIA-1 isoform p40, p40-TIA-1

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

43kD

Rabbit

Recombinant Human TIA1 (Asp104-Trp181).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.