Major cellular 3''-to-5'' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3'' termini . Prevents cell-intrinsic initiation of autoimmunity . Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements . Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation . Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation . Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon . Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates . Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light . During GZMA-mediated cell death, contributes to DNA damage in concert with NME1 . NME1 nicks one strand of DNA and TREX1 removes bases from the free 3'' end to enhance DNA damage and prevent DNA end reannealing and rapid repair . .

q9nsu2

Three-prime repair exonuclease 1 (EC 3.1.11.2) (3''-5'' exonuclease TREX1) (Deoxyribonuclease III) (DNase III),TREX1,TREX1, Deoxyribonuclease III, DNase III, Three-prime repair exonuclease 1, 3''-5'' exonuclease TREX1

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

34kD

Rabbit

Recombinant Human TREX1 (Met11-His222).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.