E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or autophagy . Ubiquitinates PIK3C2B and inhibits its activity by mediating the formation of ''Lys-48''-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of ''Lys-63''-linked polyubiquitin chains at ''Lys-220'' of WASHC1, leading to promote endosomal F-actin assembly . Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. Forms a complex with and ubiquitinates the ubiquitin-specific protease USP7, which in turn deubiquitinates RIPK1 resulting in the positive regulation of TNF-alpha-induced apoptosis . In addition, acts with USP7 or PTPN11 as an inhibitor of the antiviral signaling pathway by promoting kinase TBK1 ubiquitination and degradation . Acts as a negative regulator of NOD2 signaling by mediating ubiquitination of NOD2, promoting its degradation by the proteasome . Alternatively, facilitates mitophagy via stabilization of active TBK1 . Negatively regulates autophagy flux under basal conditions by directly polyubiquitinating ULK1 . During starvation-induced autophagy, catalyzes non-degradative ubiquitination of the kinase STK38L promoting its activation and phosphorylation of ULK1 leading to its ubiquitination and degradation to restrain the amplitude and duration of autophagy . .; (Microbial infection) Positively regulates hepatitis C virus replication by suppressing type I IFN response during infection. .

p14373

Zinc finger protein RFP (EC 2.3.2.27) (RING finger protein 76) (Ret finger protein) (Tripartite motif-containing protein 27),TRIM27 RFP RNF76,RING finger protein 76, Zinc finger protein RFP, RFP, TRIM27, RNF76, RING-type E3 ubiquitin transferase TRIM27, Tripartite motif-containing protein 27, Ret finger protein

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

59kD

Rabbit

Recombinant Human TRIM27 (Ser315-Pro513).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.