E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy . Plays a role in virus-triggered induction of IFN-beta and TNF-alpha by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for ''Lys-63''-linked ubiquitination which promotes the interaction of STING1 with TBK1 . Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages . Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner . Plays an essential role in oxidative stress induced cell death by inducing loss of transmembrane potential and enhancing mitochondrial reactive oxygen species (ROS) production during oxidative stress conditions . Ubiquitinates XIAP and targets it for proteasomal degradation . Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation . May ubiquitinate BBS2 . Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha (By similarity). Also acts as a regulator of autophagy by mediating formation of unanchored ''Lys-63''-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation . Positively regulates dendritic branching by promoting ubiquitination and subsequent degradation of the epigenetic factor CDYL . Under metabolic stress and phosphorylation by CHK2, mediates ''Lys-63''-linked ubiquitination of ATG7 at ''Lys-45'' to initiate autophagy . .; (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo . Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo . .

q13049

E3 ubiquitin-protein ligase TRIM32 (EC 2.3.2.27) (72 kDa Tat-interacting protein) (RING-type E3 ubiquitin transferase TRIM32) (Tripartite motif-containing protein 32) (Zinc finger protein HT2A),TRIM32 HT2A,E3 ubiquitin-protein ligase TRIM32, 2.3.2.27, 72 kDa Tat-interacting protein, RING-type E3 ubiquitin transferase TRIM32, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

72kD

Rabbit

Recombinant Human TRIM32 (Ser7-Arg101).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.