Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection . Also plays an important role in neutrophil activation . Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells . G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways . .

p10145

Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I); Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b); IL-8(5-77); IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF); IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV); IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V); IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)],CXCL8 IL8,GCP/IL-8 protein II,MDNCF,IL8/NAP1 form III,IL-8(1-77),Neutrophil-activating factor,GCP/IL-8 protein I,GCP/IL-8 protein VI,Interleukin-8,Chemokine (C-X-C motif) ligand 8,Neutrophil-activating protein 1,Monocyte-derived neutrophil chemotactic factor,(Ser-IL-8)72,MDNCF-b,LYNAP,GCP/IL-8 protein V,IL8/NAP1 form IV,MONAP,IL8/NAP1 form V,IL8/NAP1 form VI,NAP-1,Granulocyte chemotactic protein 1,GCP/IL-8 protein III,IL8/NAP1 form II,IL8,IL-8,CXCL8,IL8/NAP1 form I,(Ala-IL-8)77,Protein 3-10C,GCP/IL-8 protein IV,Emoctakin,GCP-1,C-X-C motif chemokine 8,NAF,T-cell chemotactic factor,MDNCF-c,Monocyte-derived neutrophil-activating peptide,Lymphocyte-derived neutrophil-activating factor

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

12kD

Rabbit

Recombinant Human IL8/CXCL8 (Ala23-Ser99).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.