Mediates the entry of L.monocytogenes into normally non-phagocytic mammalian host cells (Probable) . Its host receptor is hepatocyte growth factor receptor (HGF receptor, a tyrosine kinase, MET) which is tyrosine-phosphorylated in response to InlB in human, green monkey, mouse and dog cell lines . Downstream adapter proteins GAB1 and CBL are phosphorylated in response to InlB, which also causes cell colony scattering . InlB binding to mammalian cells is saturable and inhibited by EDTA; InlB-coated beads can be taken up by host cells . Complement component 1 Q subcomponent-binding protein (gC1q-R, C1QBP) might act as an InlB receptor, leading to activation of PI3-kinase in green monkey cells . Stimulation of Tyr-phosphorylation by InlB is antagonized by C1QBP, showing that potentiation of MET signaling via the GW domains is not mediated by C1QBP; the exact role of C1QBP remains to be determined . Stimulation of Tyr-phosphorylation of MET by InlB is potentiated by the InlB GW domains and glycosaminoglycans such as heparin; exogenously added InlB, or hepatocyte growth factor (HGF) will also substitute for bacterial InlB, suggesting InlB promotes bacterial invasion by mimicking the hormone HGF . May stimulate phosphatidylinositol 4,5-bisphosphate 3-kinase (PI3-kinase) in green monkey cells, has less effect in humans as PI3-kinase is constitutively and highly expressed in Caco cells (Probable). Binds heparin; C1QBP and heparin seem to bind to the GW domains . .

p0dqd3

Internalin B (InlB) (Invasion protein InlB),inlB,Internalin B, InlB, Invasion protein InlB, inlB

Listeria monocytogenes serotype 1/2a (strain EGD / Mackaness)

ELISA

ELISA 1:5000-1:20000

72kD

Alpaca

Monoclonal

SA243

VHH-8His-Cys-tag

Purified by Nickel column.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.