Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi . The PAMPs notably include mycobacterial trehalose 6,6''-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions . Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells . Binding of mycobacterial trehalose 6,6''-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 (Th1) and T-helper 17 (Th17) cell subtypes . Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation . Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue . .

q9r0q8

C-type lectin domain family 4 member E (C-type lectin superfamily member 9) (Macrophage-inducible C-type lectin) (Mincle),Clec4e Clecsf9 Mincle,C-type lectin superfamily member 9, Macrophage-inducible C-type lectin, MINCLE, CLECSF9, CLEC4E, C-type lectin domain family 4 member E

Mouse

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

25kD

Rabbit

Recombinant Mouse CLEC4E (Glu65-Asp214).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.