[Ninjurin-1]: Effector of various programmed cell death, such as pyroptosis and necroptosis, which mediates plasma membrane rupture (cytolysis) . Oligomerizes in response to death stimuli and forms ring-like structures on the plasma membrane: acts by cutting and shedding membrane disks, like a cookie cutter, leading to membrane damage and loss that cannot be repaired by the cell . Plasma membrane rupture leads to release intracellular molecules named damage-associated molecular patterns (DAMPs) that propagate the inflammatory response . Mechanistically, mediates plasma membrane rupture by introducing hydrophilic faces of 2 alpha helices into the hydrophobic membrane . Induces plasma membrane rupture downstream of Gasdermin (GSDMA, GSDMB, GSDMC, GSDMD, or GSDME) or MLKL during pyroptosis or necroptosis, respectively . Acts as an effector of PANoptosis downstream of CASP1, CASP4, CASP8 and RIPK3 . Also induces plasma membrane rupture in response to cell swelling caused by osmotic stress and ferroptosis downstream of lipid peroxidation . Acts as a regulator of Toll-like receptor 4 (TLR4) signaling triggered by lipopolysaccharide (LPS) during systemic inflammation; directly binds LPS . Involved in leukocyte migration during inflammation by promoting transendothelial migration of macrophages via homotypic binding . Promotes the migration of monocytes across the brain endothelium to central nervous system inflammatory lesions (By similarity). Also acts as a homophilic transmembrane adhesion molecule involved in various processes such as axonal growth, cell chemotaxis and angiogenesis . Promotes cell adhesion by mediating homophilic interactions via its extracellular N-terminal adhesion motif (N-NAM) . Involved in the progression of the inflammatory stress by promoting cell-to-cell interactions between immune cells and endothelial cells . Plays a role in nerve regeneration by promoting maturation of Schwann cells . Acts as a regulator of angiogenesis . Promotes the formation of new vessels by mediating the interaction between capillary pericyte cells and endothelial cells . Also mediates vascular functions in penile tissue as well as vascular formation . Promotes osteoclasts development by enhancing the survival of prefusion osteoclasts . Also involved in striated muscle growth and differentiation . Also involved in cell senescence in a p53/TP53 manner, possibly by acting as an indirect regulator of p53/TP53 mRNA translation . .; [Secreted ninjurin-1]: Secreted form generated by cleavage, which has chemotactic activity . Acts as an anti-inflammatory mediator by promoting monocyte recruitment, thereby ameliorating atherosclerosis . .

o70131

Ninjurin-1 (Nerve injury-induced protein 1) [Cleaved into: Secreted ninjurin-1 (Soluble ninjurin-1) (sNinJ1)],Ninj1,Ninjurin-1, Nerve injury-induced protein 1, Secreted ninjurin-1, Soluble ninjurin-1, NINJ1

Mouse

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

17kD

Rabbit

Recombinant Mouse NINJ1 (Glu2-Pro79 & Gln142-Gln152).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.