Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis . The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases . The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage . Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3''-5'' exonuclease activity . After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection . MRE11 first endonucleolytically cleaves the 5'' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR . It then generates a single-stranded DNA gap via 3'' to 5'' exonucleolytic degradation to create entry sites for EXO1- and DNA2-mediated 5'' to 3'' long-range resection, which is required for single-strand invasion and recombination . RBBP8/CtIP specifically promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends . MRE11 endonuclease activity is also enhanced by AGER/RAGE (By similarity). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR . The MRN complex is also required for the processing of R-loops . The MRN complex is involved in the activation of the cGAS-STING pathway induced by DNA damage during tumorigenesis: the MRN complex acts by displacing CGAS from nucleosome sequestration, thereby activating it (By similarity). In telomeres the MRN complex may modulate t-loop formation . .; MRE11 contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). .

p49959

Double-strand break repair protein MRE11 (EC 3.1.-.-) (Meiotic recombination 11 homolog 1) (MRE11 homolog 1) (Meiotic recombination 11 homolog A) (MRE11 homolog A),MRE11 HNGS1 MRE11A,HNGS1,Double-strand break repair protein MRE11,MRE11A,Double-strand break repair protein MRE11A,Meiotic recombination 11 homolog A,MRE11 homolog A,MRE11 homolog 1,MRE11,Meiotic recombination 11 homolog 1

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

81kD

Rabbit

Recombinant Human MRE11 (Pro375-Lys609).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.