Functions in inorganic phosphate uptake, although probably not the main uptake protein under phosphate starvation . Binds phosphate; probably able to bind both H(2)PO(4)(-) and HPO(4)(2-) . Part of the ABC transporter complex PstSACB involved in phosphate import (Probable). .; A host TLR2 agonist (toll-like receptor), shown experimentally for human and mouse . Requires both host TLR1 and TLR2 as coreceptors to elicit host response in mouse (TLR6 may also play a role) neither CD14 or CD36 function as accessory receptors . Protein purified from culture filtrate induces host (human) monocytes to produce TNF-alpha, IL-6 and IL-12 p40 (IL12B) via ERK1/2 (MAPK3 and MAPK1) and p38 MAPK pathways; MEK inhibitors U0126 and PD98059 and p38 inhibitor SB203580 block most cytokine production . Host ERK1/2 and p38 MAPK activation is mediated mainly by TLR2, but also partially by TLR4, and unlike the case for lipoprotein LpqH the protein moiety of PstS1 seems to be the antigenic agent . Greater activation of ERK1/2 and p38 MAPK is seen in patients with active pulmonary tuberculosis than in tuberculin-negative patients . Induces apoptosis when incubated with human monocyte-derived macrophages via TLR2 . Protein purified from culture filtrate acts via TLR2 and TLR4 to induce host macrophage (shown for mouse) endoplasmic reticulum stress-mediated apoptosis via MAPK (at least JNK), C-C motif chemokine 2 (MCP-1, Ccl2) and ZC3H12 endoribonucleases (MCPIP, Zc3h12) . Functions as an adhesin, binds to human and mouse macrophages via mannose residues, binds to the mouse macrophage mannose receptor (possibly Mrc1) and mediates bacterial phagocytosis . .

p9wgu1

Phosphate-binding protein PstS 1 (PBP 1) (PhoS1) (PstS-1) (38-kDa glycolipoprotein) (38-kDa lipoprotein) (P38) (Antigen Ag78) (Protein antigen B) (Pab),pstS1 phoS1 Rv0934 MTCY08D9.05c,Phosphate-binding protein PstS 1, PBP 1, PhoS1, PstS-1, 38-kDa glycolipoprotein, 38-kDa lipoprotein, P38, Antigen Ag78, Protein antigen B, Pab, pstS1, phoS1, Rv0934, MTCY08D9.05c

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

FC,ELISA,SPR

FC 1:50-1:200
ELISA 1:5000-1:20000
SPR,The optimal dilutions should be determined by the end user.

39kD

Human

Monoclonal

SA414

IgG1

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.