Hydrolase that can specifically remove ''Lys-48''-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation . Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen . Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy . Isoform 1 destabilizes RNF128, leading to prevent anergy . In contrast, isoform 2 stabilizes RNF128 and promotes anergy . Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128 . Deubiquitinates estrogen receptor alpha (ESR1) . Mediates deubiquitination of ''Lys-48''-linked polyubiquitin chains, but not ''Lys-63''-linked polyubiquitin chains . Not able to cleave di-ubiquitin . Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to ''Lys-48''-linked ubiquitin . .; Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of ''Lys-63''-linked histone H2A and H2AX at DNA damage sites . Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of ''Lys-63''-linked histone H2A and H2AX marks . Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1 . The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved ''Lys48''-linked di-ubiquitin chain . Acts as a regulator of mTORC1 and mTORC2 complexes . When phosphorylated at Tyr-26, acts as an activator of the mTORC1 complex by mediating deubiquitination of RPTOR via a non-catalytic process: acts by binding and inhibiting the activity of the ubiquitin-conjugating enzyme E2 (UBE2D1/UBCH5A, UBE2W/UBC16 and UBE2N/UBC13), thereby preventing ubiquitination of RPTOR . Can also act as an inhibitor of the mTORC1 and mTORC2 complexes in response to amino acids by mediating non-catalytic deubiquitination of DEPTOR . .

q96fw1

Ubiquitin thioesterase OTUB1 (EC 3.4.19.12) (Deubiquitinating enzyme OTUB1) (OTU domain-containing ubiquitin aldehyde-binding protein 1) (Otubain-1) (hOTU1) (Ubiquitin-specific-processing protease OTUB1),OTUB1 OTB1 OTU1 HSPC263,OTU1,hOTU1,OTU domain-containing ubiquitin aldehyde-binding protein 1,Deubiquitinating enzyme OTUB1,Otubain-1,OTUB1,Ubiquitin-specific-processing protease OTUB1,Ubiquitin thioesterase OTUB1,OTB1

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

32kD

Rabbit

Recombinant Human OTUB1 (Met1-Lys271).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.