Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis . The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases . The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage . The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3''-5'' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination . Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11 . RAD50 provides an ATP-dependent control of MRE11 by positioning DNA ends into the MRE11 active site: ATP-binding induces a large structural change from an open form with accessible MRE11 nuclease sites into a closed form (By similarity). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR . The MRN complex is also required for the processing of R-loops . In telomeres the MRN complex may modulate t-loop formation . .

q92878

DNA repair protein RAD50 (hRAD50) (EC 3.6.-.-),RAD50,RAD50,hRAD50,DNA repair protein RAD50

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

154kD

Rabbit

Recombinant Human RAD50 (Thr437-Val792).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.