Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1 . In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes . In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP . Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at ''Ser-9'' and inhibiting its activity . Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex . In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation . Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at ''Ser-1798'', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway . Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR . Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function . Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4) . Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB . Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression . Phosphorylates EPHA2 at ''Ser-897'', the RPS6KA-EPHA2 signaling pathway controls cell migration . In response to mTORC1 activation, phosphorylates EIF4B at ''Ser-406'' and ''Ser-422'' which stimulates bicarbonate cotransporter SLC4A7 mRNA translation, increasing SLC4A7 protein abundance and function . .; (Microbial infection) Promotes the late transcription and translation of viral lytic genes during Kaposi''s sarcoma-associated herpesvirus/HHV-8 infection, when constitutively activated. .

q15418

Ribosomal protein S6 kinase alpha-1 (S6K-alpha-1) (EC 2.7.11.1) (90 kDa ribosomal protein S6 kinase 1) (p90-RSK 1) (p90RSK1) (p90S6K) (MAP kinase-activated protein kinase 1a) (MAPK-activated protein kinase 1a) (MAPKAP kinase 1a) (MAPKAPK-1a) (Ribosomal S6 kinase 1) (RSK-1),RPS6KA1 MAPKAPK1A RSK1,p90-RSK 1,Ribosomal S6 kinase 1,RSK1,p90RSK1,RPS6KA1,MAPKAP kinase 1a,MAPK-activated protein kinase 1a,RSK-1,MAPKAPK1A,MAP kinase-activated protein kinase 1a,MAPKAPK-1a,S6K-alpha-1,90 kDa ribosomal protein S6 kinase 1,Ribosomal protein S6 kinase alpha-1,p90S6K

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

83kD

Rabbit

Recombinant Human RPS6KA1 (Phe62-Tyr321).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.