[Capsid protein]: Capsid protein interacts with genomic RNA and assembles into icosahedric core particles 65-70 nm in diameter . The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions from host Golgi membranes . Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion . Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32 . Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells . Induces apoptosis when expressed in transfected cells . .; [Spike glycoprotein E2]: Responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. The surface glycoproteins display an irregular helical organization and a pseudo-tetrameric inner nucleocapsid arrangement . .; [Spike glycoprotein E1]: Class II viral fusion protein . Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits (By similarity). This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion . The cytoplasmic tail of spike glycoprotein E1 modulates virus release . The surface glycoproteins display an irregular helical organization and a pseudo-tetrameric inner nucleocapsid arrangement . .

p08563, p08564, p07566, q8va10, q99ie6, p19725, q9j6k8

Structural polyprotein (p110) [Cleaved into: Capsid protein (Coat protein) (C); Spike glycoprotein E2 (E2 envelope glycoprotein); Spike glycoprotein E1 (E1 envelope glycoprotein)],Polyprotein

Rubella virus (RUBV)

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

115kD

Rabbit

Recombinant Rubella virus/RUBV Poly(Asn127-Arg277).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.