Histone methyltransferase that specifically trimethylates ''Lys-9'' of histone H3. H3 ''Lys-9'' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 ''Lys-9'' trimethylation is coordinated with DNA methylation . Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone ''Lys-9'' trimethylation . Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 . SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells . Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) . In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing . The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3''-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions . .

q15047

Histone-lysine N-methyltransferase SETDB1 (EC 2.1.1.366) (ERG-associated protein with SET domain) (ESET) (Histone H3-K9 methyltransferase 4) (H3-K9-HMTase 4) (Lysine N-methyltransferase 1E) (SET domain bifurcated 1),SETDB1 ESET KIAA0067 KMT1E,H3-K9-HMTase 4,ESET,SETDB1,SET domain bifurcated 1,Histone H3-K9 methyltransferase 4,KMT1E,KIAA0067,Lysine N-methyltransferase 1E,ERG-associated protein with SET domain,Histone-lysine N-methyltransferase SETDB1

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

144kD

Rabbit

Recombinant Human SETDB1 (Met1-Ser350).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.