Heterodimer CA-CB: Crotoxin is a potent presynaptic neurotoxin that possesses phospholipase A2 (PLA2) activity and exerts a lethal action by blocking neuromuscular transmission (By similarity). It consists of a non-covalent association of a basic and weakly toxic PLA2 subunit (CBa2, CBb, CBc, or CBd), with a small acidic, non-enzymatic and non-toxic subunit (CA1, CA2, CA3 or CA4) (By similarity). The complex acts by binding to a specific 48-kDa protein (R48) receptor located on presynaptic membranes, forming a transient ternary complex CA-CB-R48, followed by dissociation of the CA-CB complex and release of the CA subunit (By similarity). At equilibrium, only the CB subunits remain associated with the specific crotoxin receptor (By similarity). In addition to neurotoxicity, crotoxin has been found to exert myotoxicity, nephrotoxicity, and cardiovascular toxicity . Moreover, anti-inflammatory, immunomodulatory, anti-tumor and analgesic effects of crotoxin have also been reported . .; Monomer CBc: The basic subunit of crotoxin is a snake venom phospholipase A2 (PLA2) that exhibits weak neurotoxicity (10-fold less than the heterodimer) and very strong anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC(50) is 0.7 nM) . In addition, it shows the same effects described for the heterodimer and binds the nucleotide-binding domain (NBD1) of CFTR chloride channels and increases the channel current . PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. .

p62022, p62023

Phospholipase A2 crotoxin basic subunit CBc (CB1) (CTX subunit CBc) (svPLA2) (EC 3.1.1.4) (Phosphatidylcholine 2-acylhydrolase),Phospholipase A2 crotoxin basic subunit CBc, CB1, CTX subunit CBc, svPLA2, 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Basic phospholipase A2 Mtx-b, svPLA2, 3.1.1.4, Mojave toxin basic chain, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 CB1, Crotalus durissus terrificus (South American rattlesnake), Crotalus scutulatus scutulatus (Mojave rattlesnake)

Crotalus durissus terrificus (South American rattlesnake), Crotalus scutulatus scutulatus (Mojave rattlesnake)

WB,IF,ICC,FC,ELISA,SPR

WB 1:500-1:2000
IF 1:100-1:500
ICC 1:100-1:400
FC 1:50-1:200
ELISA 1:5000-1:20000
SPR,The optimal dilutions should be determined by the end user.

16kD

Mouse

Monoclonal

Al246

IgG2a

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.