Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath . Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath . Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes . Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear . In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network . Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 . Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility . Plays a role in cell proliferation by regulating the G1/S-phase transition . Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 . .

o94811

Tubulin polymerization-promoting protein (TPPP) (EC 3.6.5.-) (25 kDa brain-specific protein) (TPPP/p25) (p24) (p25-alpha),TPPP TPPP1,25 kDa brain-specific protein,TPPP/p25,Tubulin polymerization-promoting protein,p24,p25-alpha,TPPP,TPPP1

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

24kD

Rabbit

Recombinant Human TPPP (Ala8-Thr210).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.