A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus . Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates . May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic . Binds penicillin . Penicillin binding is covalent, does not require lipidation, and is zinc-dependent . While this protein has beta-lactamase activity in vitro, that is probably not its role in vivo, as T.pallidum is very sensitive to penicillin antibiotics . .; A pathogen-specific membrane antigen . Most abundant of the membrane lipoproteins, only found in pathogenic treponemes, suggesting that it is an important structural moiety in the cell envelope of virulent treponemal subspecies. A lipopeptide corresponding to the first 6 mature residues induces host (human and mouse) cytokine release by monocyte cell lines via TLR2 and CD14; nonlipidated protein does not stimulate host cells . Stimulates host (human) dendritic cell maturation to become MHC class II-positive antigen presenting cells via TLR2, which depends on lipidation; nonlipidated protein does not stimulate maturation . .

p29723

Putative DD-carboxypeptidase TP_0574 (EC 3.4.-.-) (47 kDa lipoprotein) (Tp47) (Tpp47) (47 kDa membrane antigen) (47-kilodalton major integral membrane immunogen),TP_0574,Putative DD-carboxypeptidase TP_0574, 3.4.-.-, 47 kDa lipoprotein, Tp47, Tpp47, 47 kDa membrane antigen, 47-kilodalton major integral membrane immunogen, TP_0574, Treponema Pallidum (TP,Syphilis)

Treponema pallidum (strain Nichols)

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

48kD

Rabbit

Recombinant Treponema pallidum TPP47 (Cys20-Gln434).

Polyclonal

IgG

Antigen affinity purification.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.