[Isoform 1]: Mitochondrial helicase involved in mtDNA replication and repair . Might have a role in mtDNA repair . Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB . Preferentially unwinds DNA substrates with pre-existing 5''-and 3''- single-stranded tails but is also active on a 5''- flap substrate . Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand . In addition to its DNA strand separation activity, also has DNA strand annealing, DNA strand-exchange and DNA branch migration activities . .; [Isoform 2]: Lack DNA unwinding and ATP hydrolysis activities . Does not bind single-stranded or double-stranded DNA . .

q96rr1

Twinkle mtDNA helicase (EC 5.6.2.3) (Progressive external ophthalmoplegia 1 protein) (T7 gp4-like protein with intramitochondrial nucleoid localization) (T7-like mitochondrial DNA helicase) (Twinkle protein, mitochondrial),TWNK C10orf2 PEO1,PEO1, Twinkle mtDNA helicase, Progressive external ophthalmoplegia 1 protein, T7-like mitochondrial DNA helicase, TWNK, T7 gp4-like protein with intramitochondrial nucleoid localization, C10orf2, Twinkle protein, mitochondrial

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

78kD

Rabbit

Recombinant Human TWNK (Val384-Lys635).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.