Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins . Catalyzes ''Lys-11''-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis . Acts by specifically elongating ''Lys-11''-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit . Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A . In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except ''Lys-48''-linked polyubiquitination . .

q16763

Ubiquitin-conjugating enzyme E2 S (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme S) (E2-EPF) (Ubiquitin carrier protein S) (Ubiquitin-conjugating enzyme E2-24 kDa) (Ubiquitin-conjugating enzyme E2-EPF5) (Ubiquitin-protein ligase S),UBE2S E2EPF OK/SW-cl.73,Ubiquitin-protein ligase S,E2EPF,Ubiquitin-conjugating enzyme E2 S,E2-EPF,Ubiquitin-conjugating enzyme E2-EPF5,Ubiquitin-conjugating enzyme E2-24 kDa,E2 ubiquitin-conjugating enzyme S,Ubiquitin carrier protein S,UBE2S

Human, Mouse, Rat

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

24kD

Rabbit

Recombinant Human UBE2S (Met1-Ala209).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.