Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA . Required for double-strand break repair and V(D)J recombination . Also has a role in chromosome translocation . The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner . It works in the 3''-5'' direction . During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection . Binding to DNA may be mediated by XRCC6 . The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold . The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together . The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step . The XRCC5-XRRC6 dimer probably also acts as a 5''-deoxyribose-5-phosphate lyase (5''-dRP lyase), by catalyzing the beta-elimination of the 5'' deoxyribose-5-phosphate at an abasic site near double-strand breaks . XRCC5 probably acts as the catalytic subunit of 5''-dRP activity, and allows to ''clean'' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined . The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription . In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression . As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome . Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome . Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway . .

p13010

X-ray repair cross-complementing protein 5 (EC 3.6.4.-) (86 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (CTC box-binding factor 85 kDa subunit) (CTC85) (CTCBF) (DNA repair protein XRCC5) (Ku80) (Ku86) (Lupus Ku autoantigen protein p86) (Nuclear factor IV) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)),XRCC5 G22P2,Lupus Ku autoantigen protein p86,XRCC5,Thyroid-lupus autoantigen,Ku86,ATP-dependent DNA helicase 2 subunit 2,CTC85,G22P2,DNA repair protein XRCC5,86 kDa subunit of Ku antigen,TLAA,Nuclear factor IV,CTC box-binding factor 85 kDa subunit,ATP-dependent DNA helicase II 80 kDa subunit,CTCBF,Ku80,X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining),X-ray repair cross-complementing protein 5

Human

WB,IHC,ELISA

WB 1:500-1:2000
IHC 1:50-1:300
ELISA 1:5000-1:20000

83kD

Rabbit

Recombinant Human XRCC5/Ku80 (Leu384-Ile732).

Polyclonal

IgG

Purified by antigen affinity column.

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.